Preclinical models for antimicrobial compound efficacy in vitro assays
Actionable in vitro testing tools to access to the first row of sharp data
Efficacy tests : In vitro set of tools
MIC
Minimum inhibitory Concentration are defined as the lowest concentration of an antimicrobial that inhibits the visible growth of a microorganism after overnight incubation. MICs are used to determine the in vitro activity of new antimicrobials.
MBC
Minimum bactericidal Concentration are defined as the lowest concentration of antimicrobial that prevents the growth of an organism after subculture on antibiotic-free media.
TKC
Time-kill curve monitor bacterial growth and death over a range of antimicrobial concentrations over time.
Antibiogram
Based on guidelines produced by the European Committee on Antimicrobial Susceptibility Testing (EUCAST), antibiograms report how susceptible bacterial strains are to a variety of antibiotics.
Biofilm formation inhibition
Biofilms are an ensemble of microbial cells irreversibly associated with a surface. Biofilm plays crucial roles in a variety of infections. Antimicrobial candidate with putative antibiofilm properties can be analyzed on biotic and abiotic surfaces.
Extracellular vesicles
Extracellular vesicles released by bacteria represent a universal, evolutionarily conserved mechanism for intercellular communication, notably through the delivery of virulence factors or modulation of host immunity. Vibiosphen owns the know-how to produce, purify and label extracellular vesicles from Gram negative and Gram positive bacteria.
Cellular microbiology : host-pathogen interaction, adhesion / invasion assay
The analysis of the host cell-pathogen interaction is required to evaluate the impact of a new compound on host cell response and pathogen virulence. Models were developed and validated to test anti-Sars-CoV-2 innovative treatments.
